Psichecktm-2 vector
WebDec 8, 2016 · The PCR products were digested with XhoI and NotI and were then ligated into the reporter vector psiCHECKTM-2 (TWIST1 3’-UTR-8bp-deletion). The overexpression construct pFLAG-TWIST1 (pTWIST1) containing the TWIST1 protein coding region without the 3’UTR and pFLAG-CMV (empty vector, pEV) was a gift from Prof. Muh-Hwa Yang at … WebApr 15, 2024 · Fig. 2: Néel vector control by cooling in opposite magnetic fields. a Optical image and its MCD image at 280 K of the front ab plane in a 500 μm-thick crystal.
Psichecktm-2 vector
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WebBy cloning the 3’UTRs of collagen IV subunits into the psiCHECKTM-2 vector, we found that seven of the eight sites in the Col4a3–Col4a6 gene complementary to miR-29 were significantly repressed by miR-29a, b (except for the 7774–7781 of Col4a3 gene). The inhibitory efficiency of miR-29a, b on these seven sites was between 27% and 57%. WebpsiCHECK-1 and psiCHECK-2 Vectors enable monitoring of changes in expression of a target gene fused to a reporter gene. They are used for optimizing RNA interference …
WebpsiCHECK-1 and psiCHECK-2 Vectors enable monitoring of changes in expression of a target gene fused to a reporter gene. Shop Promega psiCHECK™ Vectors at … Web2 days ago · WT and mutant PABPC1L 3′UTR were cloned into the C-terminus of the Renilla luciferase gene in the psiCHECK™-2 vector. Empty vector was used as the control, and Firefly luciferase expression served as the internal standard for transfection efficiency. HeLa cell extracts were prepared 36 h after transfection and assayed for the Firefly and ...
WebPsicheck Tm 2 Dual Luciferase Reporter Vector, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, … WebpsiCHECKTM-2-vector and 50nM of the miR-876-5p or mimic-NC using ExFect®2000 Transfection Reagent (Vazyme Biotech Co., Ltd). Cell lysates were prepared using Passive Lysis Buffer (Promega) 48h after transfec-tion, and luciferase activity was measured using the Dual-Luciferase Reporter Assay (Promega). Experiments were repeated three times.
WebpsiCHECKTM-2 vector was confirmed by sanger sequencing (GATC Biotech (Konstanz, Germany)) of the psiCHECKTM-2 vector after cloning. The reverse complement of CCND1 insert with the target site is marked with yellow in the FASTA sequence, indicating a successful cloning of CCND1 target site into the psiCHECKTM-2 vector. > CCND1 …
WebFeb 8, 2024 · PsiCHECKTM‐2 vector without inserted gene was used as negative control (NC) plasmid. The inserted sequences were listed in Table. Table.1. 1. Following the procedures provided by Ribobio and Promega, A7r5 cells were co‐transfected with PsiCHECKTM‐2 vector (WT/MUT/NC) and oligonucleotides (miR‐137 mimic or mimic … boys 20 bicycle with training wheelsWebFrom the psiCHECK-2 vector (Promega, Cat. No. C8021), a 231-nucleotide (nt) region was selected from the firefly luciferase gene sequence available for (5) Tumor elimination by … gwendolyn brooks the vacant lotWebThe psiCHECK™-2 Vector is designed to provide quantitative and rapid optimization of RNA interference (RNAi). The vector enables monitoring of changes in expression of a target … gwendolyn brooks teachingWebHere we demonstrate the use of the psiCHECK™-2 Vector as a biosensor for miRNA-21 (miR-21) in different cell lines and as a tool to test an miRNA target. Generating an miRNA … gwendolyn bullockWebpsiCHECK-2 Vector Database Welcome to Vector Database! Vector database is a digital collection of vector backbones assembled from publications and commercially available … gwendolyn brown tell allWebFeb 8, 2024 · PsiCHECKTM-2 vector without inserted gene was used as negative control (NC) plasmid. The inserted sequences were listed in Table. 1. Following the procedures provided by Ribobio and Promega, A7r5 cells were co-transfected with PsiCHECKTM-2 vector (WT/MUT/NC) and oligonucleotides (miR-137 mimic or mimic control) for 48 hours. boys 22 rifleWebDec 19, 2024 · By cloning the 3’UTRs of collagen IV subunits into the psiCHECKTM-2 vector, we found that seven of the eight sites in the Col4a3–Col4a6 gene complementary to miR-29 were significantly repressed... gwendolyn bryant-smith