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Peasy-t5 zero cloning kit

WebFeb 26, 2024 · The amplicons of the secondary PCR were purified and then cloned into pEASY-T5 Zero Cloning Vector. At least three independent positive clones of each fragment were sequenced by Hangzhou Ykang Biological Technology and Service, and sequence alignment and splicing were done using DNAMAN software. Webkit (TiaP., Ltd, Beijing, China), according to the man-ufacturer’s instructions. The recovered PCR products were cloned using the pEASY-T5 Zero Cloning Kit (TransGen Biotech (Beijing) Co., Ltd, Beijing, China) by following the product manual. Two positive final clones were submitted to Invitrogen Corporation (Guangzhou, China) for sequencing.

Cloning and allelic variation of two novel catalase genes …

WebLinearized version of pUC19 for use with the GeneArt® Seamless Cloning and Assembly Kit. Linearized version of pUC19 for use with the GeneArt® Seamless Cloning and Assembly Kit. Features; Plasmids; ... pEASY-T5 Zero (linearized) pKF 18k-2. pSMART LCKan (linearized) CMV promoter. pEX-1. pKF 19k-2. pSP64 Poly(A) GAL1 promoter. pEX-A. pKOV ... WebpEASY® -Blunt Zero Cloning Kit Catalog Number：CB501-01 Price：$112.00 Specification： 20 rxns 60 rxns Quantity： Manual Vector Information MSDS COA Inquire … evoluent vertical mouse 4 small https://bulkfoodinvesting.com

PEASY-T5 Zero Cloning Kit - Beijing TransGen Biotech Co., Ltd ...

WebMar 29, 2024 · Purified PCR product was cloned with pEASY ®-T5 Zero Cloning Kit containing Trans1-T1 Phage-resistant chemically competent cells (Transgen Biotech). Twelve to 80 positive colonies per individual were selected and sequenced bidirectionally with an automatic sequencer (ABI PRISM 3730; Invitrogen Biotechnology Co., Ltd.). WebIntroduction Product Overview Description of the system The Zero Blunt® PCR Cloning Kit is designed to clone blunt PCR fragments (or any blunt DNA fragment) with a low background of nonrecombinants. The - pCR®-Blunt vector contains the lethal E. coli ccdB gene fused to the C-terminus of LacZα (Bernard et al., 1994).Ligation of a blunt PCR fragment disrupts … WebProduct Details. pEASY® -Blunt Simple Cloning Vector eliminates the multi-cloning sites of pEASY® -Blunt Cloning Vector. It is designed for cloning and sequencing Pfu-amplified PCR products. •5 minutes fast ligation of Pfu-amplified PCR products. •Kanamycin and Ampicillin resistance genes for selection. •Easy blue/white selection. br\\u0026cf phone number

CT501-02 pEASY®-T5 Zero Cloning Kit Quimigen

Category:CT501-02 pEASY®-T5 Zero Cloning Kit Quimigen

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Peasy-t5 zero cloning kit

pEASY-Blunt Zero Cloning Kit - CB501 Civic Bioscience

WebJan 8, 2016 · The recovered PCR products were cloned using the pEASY-T5 Zero Cloning Kit (TransGen Biotech Co., Ltd, Beijing, China) by following the product manual. Two positive final clones were submitted to Invitrogen Corporation (Guangzhou, China) for sequencing. Prokaryotic Expression and SDS-PAGE Analysis.

Peasy-t5 zero cloning kit

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WebMay 20, 2024 · The PCR products obtained were purified and ligated into the vector pEASY -T5 and subsequently recombinant plasmids were used to transform E. coli Trans 1-T1 competent cells using a pEASY -T5 Zero Cloning Kit (TransGen Biotech, Beijing, China). Both of the recombinant plasmids were verified by sequencing. WebJul 11, 2024 · For the sensitivity detection, plasmid pEASY-T5-MDV which contains a 407 bp target segment in the pEASY™-T5 Zero Cloning Vector was constructed and sequenced. The purified positive control pEASY-T5-MDV plasmid (4362 bp) was used as the initial template with a DNA concentration of 31.7 ng/μL. The initial copy number was about 6.7× …

WebpEASY® -T5 Zero Cloning Kit pEASY®-T5 Zero Cloning Vector contains a suicide gene. Pricing & AvailabilityDownload CatalogueAdd to wishlist View More Details Add to Cart pEASY® -Uni Seamless Cloning and Assembly Kit No restriction enzyme digestions, > 95% cloning efficiency, and no extra sequences introduced. WebOct 1, 2016 · pEASY®-T5 Zero Cloning Kit – CT501. $ 338.00 – $ 673.00 CAD. 0 in stock (expect 7-21 days for delivery) Format. You will earn 16$ if you order this product! $ …

WebThe Zero Blunt™ PCR Cloning Kit uses the multipurpose cloning vector pCR™-Blunt and ExpressLink™ T4 DNA Ligase to generate a ligation product in a five-minute, room-temperature ligation step. The Zero Blunt™ PCR Cloning Kit is designed to clone blunt PCR fragments (or any blunt DNA fragment) with a low background of non-recombinants ... WebFeatures of the pEASY-Blunt Zero Cloning Kit. 5 minutes fast insertion of blunt-end PCR products.by topoisomerase. High cloning efficiency. Positive clones up to 100%. No blue/white selection needed. Suitable for larger …

WebThe recovered PCR products were cloned using the pEASY-T5 Zero Cloning Kit (TransGen Biotech (Beijing) Co., Ltd, Beijing, China) by following the product manual. Two positive final clones were submitted to Invitrogen Corporation (Guangzhou, China) for sequencing. Taq 2X PCR Master Mix, DNA Marker, deoxyribonucleoside triphosphates (dNTPs) and ...

WebFree Trial pEASY-Blunt Simple (linearized) Cloning vector for blunt-ended PCR products, with blue-white selection, kanamycin and ampicillin resistance markers, and a T7 promoter but … evolu high chairWebMay 28, 2024 · The products were ligated to T5 zero cloning vector and transformed with Trans1-T1 into Competent cells by the heat-shock process at 42°C for 30 s (pEASY®-T5 Zero Cloning Kit). Three colonies were randomly picked from each transformation and incubated in Luria-Bertani liquid media at 37°C overnight under continuous shaking. One … evolu healthWebpEASY-T5 Zero Cloning Kit (TransGen Biotech, Beijing, China). Both of the recombinant plasmids were verified by sequencing. All the sequences of the primers used in this study are shown in br\u0026cf phone numberWebJul 30, 2024 · Recombination plasmid of HA gene of H5 subtype has been constructed using the pEASY™-T5 Zero Cloning Kit. The plasmid was cleaved using a suitable restriction enzyme. The digested plasmid was purified with a DNA recovery kit, the concentration was measured with a Nano Drop nucleic acid quantifier, and then an appropriate amount was … br type of elementWebJan 22, 2024 · The product was cloned into the pEASY-T5 Zero Cloning vector (TransGene Biotech, Beijing, China) for sequencing. For 5′ RACE, the gene specific primers were designed based on the 3′ cDNA end sequence. The 5′ RACE cDNA template was synthesized by SMARTer@RACE cDNA Amplification Kit (TaKaRa, Tokyo, Japan). evolur aurora bed railWeb关于其他常用耗材的网上超市合同公告采购信息，招标信息，机电设备采购平台 br\u0026w railroadWebpEASY ®-T5 Zero Cloning Vector contains a suicide gene. Ligation of PCR fragment disrupts the expression of the gene. Cells that contain non-recombinant vector are killed … evol unwrapped chicken egg roll bowl