Pdgfb-creert2
Splet01. jul. 2024 · PDGFRβ-P2A-CreER T2 mice allow for tamoxifen-inducible expression of Cre-recombinase under the control of the platelet derived growth factor receptor-β (PDGFRβ) … Splet29. maj 2024 · The Tgfbr2flox/flox 22, PDGFB-CreERT2 20, and Rosa26-loxSTOPlox-YFP 22 mouse strains (on a C57Bl6/129S4 genetic background)have been described elsewhere. Pups (P1-P3) were injected in the stomach ...
Pdgfb-creert2
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Splet07. jul. 2024 · For titration of lentiviruses encoding shCdkn2a-PDGFB-T2A-CreERT2, the cells were fixed with 10% formalin (acetate buffered, FisherScientific) and stained as … SpletThe targeting vector used to modify PAC 546-M3 is illustrated (a). The construct was designed to insert the tamoxifen-inducible form of Cre recombinase (CreERT2) (Indra et al., 1999) into the first coding exon of the Pdgfra gene (exon 2). Homology regions 0.5 kb in length were amplified by PCR from the genomic PAC using Expand High Fidelity ...
Splet19. feb. 2024 · We engineered these viruses to coexpress CreERT2 with PDGFB, allowing for deletion of floxed genes specifically in transduced cells, and designed another version … Splet16. nov. 2024 · Abstract. Restoring blood flow after myocardial infarction (MI) is essential for survival of existing and newly regenerated tissue. Endogenous vascular repair processes are deployed following injury but are poorly understood. We sought to determine whether developmental mechanisms of coronary vessel formation are intrinsically reactivated in ...
Splet01. maj 2024 · Pdgfb‐iCre ERT 2;JAK2 V617F/ WT mice develop a myeloproliferative disease 10 weeks after induction of JAK2V617F expression. Conditional flexed JAK2 … Splet一、品系介绍： Cre-ERT2小鼠是一类含有雌激素受体（estrogen receptor，ER）的配体结合区突变体（ERT）与Cre重组酶的融合蛋白表达的小鼠。 Cre-ERT2在无Tamoxifen诱导的 …
Splet27. jul. 2024 · PDGFRβ-P2A-Cre T2 mice (recently deposited at The Jackson Laboratory as Stock No. 030201) were generated by replacing the translation stop codon (TAG) of the …
Splet029684 - Pdgfr-β-CreER [T2] Strain Details. Technical Information Scientists. Overview. physiotherapy kenilworthSpletWe observed that Pdgfb-iCreERT2 and Cdh5(PAC)-CreERT2- mediated recombination occurs in the vast majority of blood endothelial cells in the brain, retina, lungs, kidney and … toothless httyd 3Splet29. maj 2024 · A tamoxifen-inducible transgenic mouse line, PDGFB-CreERT2, that selectively expresses Cre recombinase in vascular endothelial cells 20 was used to target a conditional type 2 TGFβ receptor allele (Tgfbr2) flanked by loxP sites (Tgfbr2flox/flox) 21. physiotherapy kendalSplet10. jan. 2013 · In this review, we described several Cre lines that target ECs, including Tie2-Cre, VE-Cadherin-CreERT2, PDGFB-CreERT2, and others. Many of these Cre alleles target all ECs, and therefore it would be helpful to generate Cre alleles and CreERT alleles that specifically target CNS ECs. To do so, one could take advantage of the split-Cre system. toothless in real lifeSpletThe PDGFRB gene provides instructions for making a protein called platelet-derived growth factor receptor beta (PDGFRβ), which is part of a family of proteins called receptor … toothless httyd pictures cartoonSplet10. jan. 2013 · In this review, we described several Cre lines that target ECs, including Tie2-Cre, VE-Cadherin-CreERT2, PDGFB-CreERT2, and others. Many of these Cre alleles target all ECs, and therefore it would be helpful to generate Cre alleles and CreERT alleles that specifically target CNS ECs. To do so, one could take advantage of the split-Cre system. toothless licks hiccup fanfiction staySplet20. jan. 2024 · The Pdgfb-CreER strain was crossed with a Nrp1-floxed strain to produce offspring with or without the CreER transgene in a homozygous Nrp1-floxed background. Upon tamoxifen treatment, Nrp1 protein was depleted in choroidal endothelial cells (arrows in Fig 1) in CreER positive (Nrp1 ΔEC) but not in CreER negative (Wt) animals ( Fig 1G–1J ). toothless licks hiccup an early morning