2024 Hu-binding site

Hu-binding site

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August undefined, 2024

HU is able to bind all forms of nucleic acids, including DNA–RNA hybrids (Balandina et al., 2002). Although HU has no specific binding motif, it is able to bind mRNA of RpoS (Balandina et al., 2001). Another study of the B. subtilis HU protein showed that HU binds an Alu domain of cytoplasmic RNA (Nakamura et al., … See more In 1975, a small, low-molecular-weight DNA-binding protein was isolated from E. coli strain U13 (Rouvière-Yaniv and Gros, 1975). At first, it was referred to as Factor U. Due to its … See more HU protein is among the most abundant and conserved nucleoid-associated proteins in eubacteria (Azam and Ishihama, 1999). The greatest abundance of HU protein has … See more Interaction of HU protein at protein level has not yet been fully explored. As mentioned above, an HU protein is able to react with another HU protein to form a dimer together … See more HU seems to be an important protein for DNA compaction, replication, transcription, recombination, and shape modulation in many bacteria (Broyles and Pettijohn, 1986; Roy et al., 2005; Oberto et al., 2009). In the coccoid … See more Web22 Mar 2011 · If the binding site for HU is estimated to be 21 bp per dimer, based on co-crystal structures , the maximum number of bound HU on λ DNA would be ~2300 dimers. …

HU binds and folds single-stranded DNA - Oxford Academic

Web29 Apr 2014 · Site-directed mutagenesis of the putative C/EBPα binding sites decreased FTO promoter activity. Overexpression and RNAi studies also indicated that C/EBP α was required for the expression of FTO. Chromatin immunoprecipitation (ChIP) experiment was carried out and the result shows direct binding of C/EBP α to the putative binding … Web3 Jun 1994 · We have recently identified one such high affinity binding site at the Mu left end by converting HU into a chemical nuclease (Lavoie and Chaconas, 1993). Using … how to request lyft with car seat

Frontiers The Identification of Metal Ion Ligand-Binding …

Initially, bacterial DNA binding proteins were thought to help stabilize bacterial DNA. Currently, many more functions of bacteria DNA binding proteins have been discovered, including the regulation of gene expression by histone-like nucleoid-structuring protein, H-NS. H-NS is about 15.6 kDa and assists in the regulation of bacterial transcription i… Webbound to its recognition site. Binding of HU is sequence un-speci¢c but, as is the case with IHF [7], binding to DNA probably involves its two arms being inserted into the minor groove. H-NS is similar in size to HU (15.6 kDa) and also binds DNA without sequence speci¢city. Unlike HU, this protein Web不废话，先介绍和使用教程，后面讲讲体验后的优缺点背景. Claude是一支由前openai的研究员和工程师组成的团队开发的新型聊天机器人，旨在对抗chatgptClaude的特点是能够检测和回避chatgpt的潜在陷阱，如逻辑错误、不恰当的内容、重复性和无聊等。 how to request lupon in prc online

The HU Regulon Is Composed of Genes Responding to …

Web3 Jun 1994 · HU-dependent DNA deformations HU wasfound to stablyoccupy the centralregion of an 83-base are thought to stimulate dnaA melting at the Escherichia pair … Web20 Dec 2007 · HU binding induces FRET between labeled ssDNA ends. Very long apparent DNA-binding sites and abnormalities in gel mobility of protein–DNA complexes often … how to request maternity leaveWeb11 Dec 2024 · In 2012, Lu et al. [ 15] used the “fragment transformation” method to predict metal ion (Ca 2+, Mg 2+, Cu 2+, Fe 3+, Mn 2+, Zn 2+) ligand binding sites, and the prediction results were obtained with a total accuracy of 94.6% and a true positive of rate 60.5%. In 2016, Hu et al. [ 16] identified four metal ions in the BioLip database by both ... how to request lwop opm

"Web15 Jun 1997 · Site-specific binding of HU is promoted by GalR binding and supercoiled DNA. Affinity cleavage mapping by chemically converting HU into a nuclease gives … " - Hu-binding site

Hu-binding site

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Web4 Jul 2013 · The dimer to base pair ratio at 50 nM HU is 1:8. This ratio can be considered as the onset of the high concentration regime. It has been reported that HU binds non-specifically to a 9 bp binding site at high concentrations of HU ( 20, 22, 31). In all aforementioned cases, 50 nM HU (1 dimer: 8 bp), a temporary stiffening is observed, … Web1 Jul 2003 · The binding site size estimated by gel mobility shift experiments for HU is variable. The mesophilic HUs from E.coli and Anabaena are predicted by gel mobility shift analyses to bind from 6 to 10 bp per dimer ( Broyles and Pettijohn, 1986; Bonnefoy and Rouviere-Yaniv, 1991; Cann et al., 1995 ).

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WebHU binding site and present evidence that protein-protein in- teractions do not mediate this high affinity binding. Further- more, we report that the mammalian HMG-1 protein … Web16 Sep 2024 · To evaluate the possible influence of the labelling site on the binding and diffusion behavior of HU on DNA, we generated a simple model of a HU–DNA complex, based on the IHF-DNA complex ...

Web3 Oct 2008 · By contrast, at high concentrations, where HU covers DNA completely, the protein assembles into a rigid superhelical filament on DNA with a persistence length several times that of naked DNA. 11 HU molecules ‘localized’ at designed binding sites in labeled oligonucleotide duplexes appear to bend DNA in solution to the same extent as that in …

Web8 Jan 2024 · Transcription factor binding sites (TFBSs) are basic components of cis-regulatory elements, which act important roles in precise regulations of gene expressions … Web1 Dec 2000 · Introduction. Escherichia coli HU, a small, basic, heat-stable DNA binding protein, is one of the most abundant proteins associated with the E.coli nucleoid (Rouviere-Yaniv and Gros, 1975; Rouviere-Yaniv, 1978). In vitro, HU shares the ability observed with histones to introduce negative supercoiling into relaxed DNA molecules in the presence …

Web19 Mar 2024 · The PWSM has been successfully used in the prediction of transcription factor binding sites and ligand binding sites (Kel et al., 2003; Hu et al., 2016a). Thus, the position weight scoring matrix (PWSM) was used to extract the position conservation information of the basic feature parameters, and the scoring matrix based on amino acid …

Web1 Nov 1996 · The structure of the NSAID binding site is also well conserved, although there are differences in its overall size and shape which may be exploited for the further … how to request mentorshipWeb26 Feb 2024 · Starting from a query sequence and predicted I-TASSER models, ATPbind can achieve an average accuracy of 72%, covering 62% of all ATP binding sites while achieving a Matthews correlation coefficient value that is significantly higher than that of other state-of-the-art predictors. Publication types Research Support, N.I.H., Extramural how to request manager for approvalWeb3 Aug 2009 · Depending on the interacting partner, the binding sites on proteins can be divided into different categories, such as DNA-binding sites, RNA-binding sites, … how to request marriage leaveWeb22 Mar 2011 · HU acts to compact DNA at physiological salt concentrations in vitro by introducing bends ranging from 53° to more than 140° in the DNA as assayed by X-ray … how to request memorialization on facebookWebHU, a major component of the bacterial nucleoid, shares properties with histones, high mobility group proteins (HMGs), and other eukaryotic proteins. HU, which participates in many major pathways of the bacterial cell, binds without sequence specificity to duplex DNA but recognizes with high affinity DNA repair intermediates. how to request mdf onlineWeb4 Feb 2009 · In these conditions, the average spacing between HU binding sites on the chromosome would amount to ∼20 kb (Supplemental Figure S2). Interestingly, the bacterial nucleoid has been described as being shaped in domains of 50–100 kb [80] whereas more recent studies re-evaluated the organization of the chromosome in 400 supercoiled … how to request medals for veteransWeb19 Jun 2008 · The histone-like HU (heat unstable) protein plays a key role in the organization and regulation of the Escherichia coli genome. The nonspecific nature of HU binding to DNA complicates analysis of the mechanism by which the protein contributes to the looping of DNA. north carolina chapel hill applicant portal