Cochlear whole mount
WebMount one cochlear turn per slide to prevent light exposure and photo bleaching during the imaging process. Place slides in a slide folder so that they lie flat. Protect from light and … WebFeb 1, 2002 · Micro Dissecting Spring Scissors used to hemisect the cochlea into its superior and inferior parts and to trim slightly the lateral wall. (We found those with 3 mm …
Cochlear whole mount
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WebAug 2, 2024 · To generate cochlear whole-mount preparations, temporal bones were dissected on ice, perfused with 4% formaldehyde and decalcified in 0.2 M EDTA for 27 h at 4°C. Once decalcification was completed, dissection of the cochlear sensory epithelium, i.e., the OC, was performed under a stereo microscope (Zeiss Stemi 200-C, … WebOct 7, 2024 · GFP expression in cochlear whole mount was analyzed and auditory brainstem response (ABR) tests were conducted one month after virus injection (for each animal, only left cochlea was injected and...
WebJan 18, 2016 · All whole mount preparations were microdissected into turns as previously described [25, 26], and the tectorial membrane was carefully removed. Cochleae were … WebThe meaning of COCHLEA is a hollow tube in the inner ear of higher vertebrates that is usually coiled like a snail shell and contains the sensory organ of hearing.
WebFeb 3, 2024 · Detailed descriptions of the surgical procedures, ABR measures and analysis, noise exposure, cochlear whole-mount preparation, immunofluorescent labeling, … WebApr 1, 2016 · Whole-mount cochlear preparations are generally positioned in such a way (with the stereocilia facing the objective) that a lateral view of the hair cells requires analysis of all images in a stack. While standard confocal software has built-in capability to view confocal stacks along the z-axis, these views are generally restricted to a single ...
WebDec 24, 2024 · Cochlear whole-mount analysis revealed loss of outer hair cells in the 50 mg/kg RV and CXP groups and partial prevention of these cells in the 0.5 mg/kg RV + CXP group. The mRNA expressions of NFκB, IL6, and IL1β were increased in the 50 mg/kg RV and CXP groups compared to the control group.
WebMar 5, 2013 · Mouse Cochlear Whole Mount Immunofluorescence. This protocol comprises the entire process of immunofluorescence staining on mouse cochlea whole … long term weather forecast brooms headWebJun 29, 2016 · Cochlear whole mount in situ hybridization: identification of longitudinal and radial gradients. Brain Res Brain Res Protoc 9: 65-76 . Google Scholar Lewin GR, Barde YA. 1996. Physiology of the neurotrophins. Annu Rev Neurosci 19: 289-317 . Google Scholar Liberman MC. 1980. long term weather forecast boulderlong term weather forecast bay of plentyWebAug 2, 2024 · Cochlear Whole-Mount Preparation. To generate cochlear whole-mount preparations, temporal bones were dissected on ice, perfused with 4% formaldehyde and decalcified in 0.2 M EDTA for 27 h at 4°C. Once decalcification was completed, dissection of the cochlear sensory epithelium, i.e., the OC, was performed under a stereo microscope … long term weather forecast brompton on swaleWebDec 9, 2024 · Immunohistochemistry on cochlear whole-mount preparations. Cochlear whole-mounts were rehydrated in a descending methanol/PBS series. For the first permeabilization step, whole-mounts were treated ... long-term weather forecast californiaWebThe cochlear “supporting cells” are an organization of heterogeneous non-sensory cell types that line the scala media (we exclude from consideration here those cells of the spiral ligament) and are sensitive to the local cochlear micro … long term weather forecast alpsWebCochlear Whole-Mount Preparation Isolated mice and rat cochleae were perfused with 4% paraformaldehyde and kept overnight at 4°C in the same solution for fixation. Cochleae were then decalcified in 100 mM EDTA … long term weather forecast boise id