WebMay 18, 2024 · CAMYEL assays involving pharmacological CB 1 receptor knockdown with AM6544 were performed as noted above, except that an AM6544 serial dilution series was prepared in serum-free medium supplemented with 1 mg·mL −1 BSA and applied to cells for 1 h. WebA retroviral vector for introducing the CAMYEL (cAMP sensor using YFP-Epac-RLuc) into mammalian cells. It has been used to facilitate studies of cAMP regulation using BRET (bioluminescence resonance energy transfer) by quantitatively and rapidly monitoring intracellular concentration of cAMP in vivo. Vector features: 5'LTR: 209-760; viral …
Characterizing Gs- and Gi-Dependent Regulation of cAMP …
WebApr 6, 2024 · Table 3.1 Binding affinity of cAMP analogs, as assessed by CAMYEL.... Figure 3.1 The CAMYEL Assay..... Figure 3.2 CAMYEL activation is induced by Epac agonists in a concentration-dependent manner..... Figure 3.3 CAMYEL can assess if cAMP analogs are agonists, super ... WebJun 22, 2024 · We have developed a synthetic method for selective functionalization of the unexplored C11 position of the MG scaffold (C6 position in indole numbering) via the use of an indole-ethylene glycol... ontario reading levels by grade
Real‐time examination of cAMP activity at relaxin family …
WebCAMYEL Assay to Study cAMP Regulation . 7 . Ligand induced cAMP regulation by GPCRs has been dynamically monitored using a BRET sensor for cAMP, CAMYEL (cAMP sensor using YFP-Epac-Rluc) (Jiang et al., 2007; Matthiesen and Nielsen, 2011). CAMYEL is composed of a cAMP binding protein, Epac WebRead how the CAMYEL BRET biosensor can be used to study the associated signaling pathways in plate readers. ... Assay Principle. The CAYMEL BRET biosensor 3 is composed of the EPAC protein bound to both a luciferase (RLuc) and yellow fluorescent protein (YFP) as shown in fig. 1. In the absence of cAMP, the conformation of EPAC … WebFeb 9, 2024 · The CAMELS Approach. 09 Feb 2024. CAMELS is a widely used approach to analyze a bank. In this context, a bank is an entity that primarily takes deposits and … ontario real estate association forms